Optimized DNA Extraction from Plants

Plant genomic DNA extraction Kit (GeNei), CTAB DNA extraction method by Porebski et al. [4 ], J. J. Doyle and J. L. Doyle [19 ], and Saghai-Maroof et al. [20 (link)] were employed for extracting DNA from the study plants. Among all the tested protocols, Saghai-Mahroof method yielded convincing results. Therefore, this method was taken and optimized for DNA extraction by varying the concentration of Tris-HCl, NaCl (Sodium Chloride), β-mercaptoethanol, and PVP (PolyVinyl Pyrrolidone).

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Sahu S.K., Thangaraj M, & Kathiresan K. (2012). DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. ISRN Molecular Biology, 2012, 205049.

Publication 2012

Ctab Genomic Mercaptoethanol Plants Polyvinyl pyrrolidone Sodium chloride Tris

Corresponding Organization :

Other organizations : Annamalai University

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Protocol cited in 10 other protocols

Variable analysis

independent variables

Concentration of Tris-HCl
Concentration of NaCl (Sodium Chloride)
Concentration of β-mercaptoethanol
Concentration of PVP (PolyVinyl Pyrrolidone)

dependent variables

DNA extraction yield

control variables

Plant genomic DNA extraction Kit (GeNei)
CTAB DNA extraction method by Porebski et al. [4]
J. J. Doyle and J. L. Doyle [19]
Saghai-Maroof et al. [20]

positive controls

Saghai-Mahroof method

negative controls

Not specified

Annotations

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