Antibody Detection of TRPC Ion Channels

These methods were previously described in detail [18 (link)]. Rabbit antibodies against TRPC6 (ACC-017) and TRPC3 (ACC-016) were obtained from Alomone Labs (Jerusalem, Israel). A mouse monoclonal antibody against TRPC5 was obtained from NeuroMAB (Davis, CA, USA). All TRPC antibodies were used at a dilution of 1:1000. The antibody against vimentin (cloneV9) was from DAKO (Agilent, Santa Clara, CA, USA) and used at a dilution of 1:1000. The antibody against NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) was obtained from LSBio (Seattle, WA, USA), and was used at a dilution of 1:1000.