Substrate specificity was studied using the 0.25 mM chromogenic substrates Z-Phe-Arg-pNA (where Z = benzyloxycarbonyl), Z-Arg-Arg-pNA (both from Bachem, Switzerland), Glp-Phe-Gln-pNA, Glp-Phe-Leu-pNA, Glp-Phe-Ala-pNA, and Glp-Val-Ala-pNA, as described in Section 4.4. The substrates Glp-Phe-Leu-pNA, Glp-Val-Ala-pNA, Glp-Phe-Ala-pNA, Glp-Phe-Ala-AMC, and Glp-Phe-Gln-pNA were synthesized, as described in [41 (link),43 (link),47 ].
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