Antibody-based Protein Expression Analysis

The following antibodies were used: anti-PLK4 (Rabbit, 1:1,000), and anti-PCNA (Mouse, 1:1,000) from Abcam (Cambridge, the United Kingdom); anti-p-p38 (Rabbit, 1:1,000), anti-p38 (Rabbit, 1:1,000), anti-p-ERK (Rabbit, 1:4,000), anti-ERK (Rabbit, 1:1,000), anti-Beclin1 (Rabbit, 1:1000), anti-Atg5 (Rabbit, 1:1000), anti-LC3B (Rabbit, 1:1000) and anti-SQSTM1/p62 (Rabbit, 1:1000) from Cell Signaling Technology (Danvers, USA); anti-caspase 3 (Rabbit, 1:500) from Proteintech (Chicago, IL, USA); anti-Bax (Rabbit, 1:1000) and anti-GAPDH (Mouse, 1:1000), anti-CDK4 (Mouse, 1:1000), anti-CDK6 (Rabbit, 1:1000) from Bioss (Beijing, China). The steps for WB were based on previously described methods17 (link).

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Tian X., He Y., Qi L., Liu D., Zhou D., Liu Y., Gong W., Han Z., Xia Y., Li H., Wang J., Zhu K., Chen L., Guo H, & Zhao Q. (2023). Autophagy Inhibition Contributes to Apoptosis of PLK4 Downregulation-induced Dormant Cells in Colorectal Cancer. International Journal of Biological Sciences, 19(9), 2817-2834.

Publication 2023

anti-PLK4 anti-PCNA anti-p-p38 anti-p38 anti-p-ERK anti-ERK anti-Beclin1 anti-Atg5 anti-LC3B anti-SQSTM1/p62 anti-caspase 3 anti-Bax anti-GAPDH anti-CDK4 anti-CDK6

Antibodies Beclin1 Caspase 3 Cdk6 Gapdh Mouse Pcna Rabbit

Corresponding Organization :

Other organizations : Tianjin Medical University Cancer Institute and Hospital

Top 5 similar protocols

Variable analysis

independent variables

Anti-PLK4 (Rabbit, 1:1,000)
Anti-PCNA (Mouse, 1:1,000)
Anti-p-p38 (Rabbit, 1:1,000)
Anti-p38 (Rabbit, 1:1,000)
Anti-p-ERK (Rabbit, 1:4,000)
Anti-ERK (Rabbit, 1:1,000)
Anti-Beclin1 (Rabbit, 1:1000)
Anti-Atg5 (Rabbit, 1:1000)
Anti-LC3B (Rabbit, 1:1000)
Anti-SQSTM1/p62 (Rabbit, 1:1000)
Anti-caspase 3 (Rabbit, 1:500)
Anti-Bax (Rabbit, 1:1000)
Anti-GAPDH (Mouse, 1:1000)
Anti-CDK4 (Mouse, 1:1000)
Anti-CDK6 (Rabbit, 1:1000)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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