Cells fixed with 1% formaldehyde were subjected to ChIP as previously described6 (link) with oligonucleotides as listed in Supplementary Table 2 . The following histone antibodies were used: anti-H3K36me3 (Abcam ab9050), anti-acetyl H4 (Millipore 06–598), anti-acetyl H3 (Millipore 06–599), anti-myc (BioLegend 626802) and anti-H3 (Abcam ab1791). Except for H3 acetylation, all ChIP-seqs included S. pombe “spike-in” added at 10% relative to Saccharomyces cerevisiae chromatin.
Precipitated DNAs were analyzed by quantitative real-time PCR using THUNDERBIRD® SYPR qPCR Mix (TOYOBO) and CFX96 cycler (Bio-Rad). The DNA libraries for ChIP-seq were prepared using Accel-NGS 2 S Plus DNA Library Kit (Swift Biosciences) and sequenced on the HiSeq2500 platform (Illumina) following the manufacturer’s instructions.
Precipitated DNAs were analyzed by quantitative real-time PCR using THUNDERBIRD® SYPR qPCR Mix (TOYOBO) and CFX96 cycler (Bio-Rad). The DNA libraries for ChIP-seq were prepared using Accel-NGS 2 S Plus DNA Library Kit (Swift Biosciences) and sequenced on the HiSeq2500 platform (Illumina) following the manufacturer’s instructions.
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