Localization of KmYME in K. marxianus

Localization of KmYME in K. marxianus was conducted as described previously (Mo et al., 2016 (link)). Imaging was conducted using a Perkin Elmer spinning-disk confocal microscope (PerkinElmer, Inc., Norwalk, CT, United States) equipped with a Zeiss PlanApo 100X/1.4 NA objective and a Photometrics EMCCD camera Evolve 51234. Images were captured by the DeltaVision softWoRx software (Applied Precision) and processed by deconvolution and z-stack projection. All images were analyzed using ImageJ.

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Wu D., Wang D, & Hong J. (2020). Effect of a Novel Alpha/Beta Hydrolase Domain Protein on Tolerance of K. marxianus to Lignocellulosic Biomass Derived Inhibitors. Frontiers in Bioengineering and Biotechnology, 8, 844.

Publication 2020

spinning-disk confocal microscope DeltaVision softWoRx software

Confocal microscope

Corresponding Organization :

Other organizations : University of Science and Technology of China, Hefei National Center for Physical Sciences at Nanoscale

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Variable analysis

independent variables

Localization of KmYME in K. marxianus

dependent variables

Imaging of KmYME localization

control variables

Perkin Elmer spinning-disk confocal microscope
Zeiss PlanApo 100X/1.4 NA objective
Photometrics EMCCD camera Evolve 51234
DeltaVision softWoRx software (Applied Precision)
Deconvolution and z-stack projection
ImageJ for image analysis

controls

No positive or negative controls were explicitly mentioned in the provided information.

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