Measurement of Macrophage Cholesterol Oleate

Adherent macrophages were incubated for 18 h with culture medium containing 10 μg/ml human oxidised LDL in the presence of 0.1 mmol/l [³H]oleate conjugated with BSA [13 (link), 14 (link)]. Cellular lipids were extracted and the radioactivity of cholesterol [³H]oleate was determined by thin-layer chromatography [13 (link), 14 (link)].

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Nagashima M., Watanabe T., Terasaki M., Tomoyasu M., Nohtomi K., Kim-Kaneyama J., Miyazaki A, & Hirano T. (2011). Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice. Diabetologia, 54(10), 2649-2659.

Publication 2011

Cellular Cholesterol Culture medium Human Ldl 1 Lipids Macrophages Oleate Radioactivity Thin layer chromatography

Corresponding Organization :

Other organizations : Showa University, Tokyo University of Pharmacy and Life Sciences

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Protocol cited in 6 other protocols

Variable analysis

independent variables

Concentration of human oxidized LDL (10 μg/ml)

dependent variables

Cellular lipid content (specifically, the radioactivity of cholesterol [³H]oleate)

control variables

Incubation time (18 h)
Concentration of [³H]oleate conjugated with BSA (0.1 mmol/l)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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