Comprehensive Stem Cell Characterization

Human Embryonic Stem Cell Marker Panel (Hicks et al., 2020 (link); Roth et al., 2020 (link); Bomba et al., 2021 (link); Osnato et al., 2021 (link)) (Abcam, ab238602) was used to analyze pluripotency protein markers by immunofluorescence. A Trilineage Differentiation Kit (Ward et al., 2017 (link)) (STEMCELL, #05230) was used to induce differentiation into the three germ layers. Human three Germ Layer 3-Color Immunocytochemistry Kit (Han et al., 2020 (link)) (R&D, SC022) was used to identify specific protein markers of the three germ layers. A Giemsa Stain solution (Solarbio, G1015) was used to analyze the karyotype. Fluorescence images were obtained under an LSM900 laser scanning confocal microscope (Zeiss, Germany).

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Gu Z., Yin Z., Song P., Wu Y., He Y., Zhu M., Wu Z., Zhao S., Huang H., Wang H., Tong C, & Qi Z. (2022). Safety and biodistribution of exosomes derived from human induced pluripotent stem cells. Frontiers in Bioengineering and Biotechnology, 10, 949724.

Publication 2022

Trilineage Differentiation Kit Human three Germ Layer 3-Color Immunocytochemistry Kit Giemsa Stain solution LSM900 laser scanning confocal microscope

Confocal laser scanning microscope Fluorescence Germ layers Giemsa stain Hicks Human Human embryonic stem cell Immunocytochemistry Immunofluorescence Karyotype Protein Stemcell

Corresponding Organization : Guangxi University

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Variable analysis

independent variables

Human Embryonic Stem Cell Marker Panel (Hicks et al., 2020; Roth et al., 2020; Bomba et al., 2021; Osnato et al., 2021) (Abcam, ab238602) was used to analyze pluripotency protein markers by immunofluorescence
A Trilineage Differentiation Kit (Ward et al., 2017) (STEMCELL, #05230) was used to induce differentiation into the three germ layers
Human three Germ Layer 3-Color Immunocytochemistry Kit (Han et al., 2020) (R&D, SC022) was used to identify specific protein markers of the three germ layers
A Giemsa Stain solution (Solarbio, G1015) was used to analyze the karyotype

dependent variables

Pluripotency protein markers analyzed by immunofluorescence
Differentiation into the three germ layers
Specific protein markers of the three germ layers
Karyotype analysis

control variables

Fluorescence images were obtained under an LSM900 laser scanning confocal microscope (Zeiss, Germany)

positive controls

No positive controls were explicitly mentioned

negative controls

No negative controls were explicitly mentioned

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