Nucleic Acid Determination of Phage

Following the manufacturer’s instructions, DNAse I, RNAse A, and mung bean nuclease (Takara Biomedical Technology Co., Ltd., Beijing, China) were used to confirm the type of nucleic acid in the phage (Kumar et al. 2021 (link)).
Phage DNA was obtained from the purified phage suspension using a viral RNA/DNA extraction kit (Takara Biomedical Technology Co., Ltd., Beijing, China) according to the manufacturer’s instructions.

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Han G., Zhang J., Luo Z., Lu B., Zhang P., Yong K., Wang Y., Luo Y., Yang Z., Ren M., Cao S, & Yao X. (2022). Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31). International Microbiology, 26(2), 327-341.

Publication 2022

DNAse I RNAse A mung bean nuclease viral RNA/DNA extraction kit

Biomedical technology Dnase i Mung bean nuclease Nucleic acid Phage Rnase Viral dna Viral rna

Corresponding Organization :

Other organizations : Sichuan Agricultural University

Top 5 similar protocols

Variable analysis

independent variables

Using DNAse I, RNAse A, and mung bean nuclease to confirm the type of nucleic acid in the phage

dependent variables

Type of nucleic acid in the phage

control variables

Following the manufacturer's instructions when using DNAse I, RNAse A, and mung bean nuclease
Using a viral RNA/DNA extraction kit to obtain phage DNA from the purified phage suspension, following the manufacturer's instructions

controls

Positive control: Not mentioned
Negative control: Not mentioned

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