Protocol detail

Generating Transgenic and Knockout Mice

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Wildtype C57BL/6 (B6) mice and MHC class II deficient (MHC-II^-/-) mice were obtained from The Jackson Laboratory (Bar Harbor, ME). MyD88^-/- mice were obtained from Mutant Mouse Resource and Research Centers. Jα18(-10)^-/- mice (hereafter referred to as Jα18^-/- mice) were generated on the B6 background in Dr. Laurent Gapin’s lab (University of Colorado) (27 (link)). We backcrossed these Jα18^-/- mice to B6 mice in our mouse colony to generate wild-type littermate controls. CD1d^-/- mice were generated in house and have been backcrossed onto B6 background for at least 12 generations (28 (link)). MR1^-/- mice were provided by Dr. Ted Hansen (Washington University) and backcrossed to B6 mice to generate MR1^+/+ littermate controls. Vα3.2⁺Vβ9⁺ CD1d-autoreactive transgenic mice (24αβ) were provided by Dr. Suzanna Cardell (University of Gothenburg) (29 (link)). MHC-II^-/-CD1d^-/- mice were generated by crossing CD1d^-/- mice and MHC-II^-/- mice. Naïve mice were housed in a specific pathogen-free facility.

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Genardi S., Visvabharathy L., Cao L., Morgun E., Cui Y., Qi C., Chen Y.H., Gapin L., Berdyshev E, & Wang C.R. (2020). Type II Natural Killer T Cells Contribute to Protection Against Systemic Methicillin-Resistant Staphylococcus aureus Infection. Frontiers in Immunology, 11, 610010.

Publication 2020

C57BL/6 (B6) mice MHC-II-/-) mice

Cd1d Mhc class ii Mice Mice mice Specific pathogen free Transgenic mice

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Variable analysis

independent variables

Genotype (Wildtype C57BL/6 (B6) mice, MHC class II deficient (MHC-II-/-) mice, MyD88-/- mice, Jα18-/- mice, CD1d-/- mice, MR1-/- mice, Vα3.2+Vβ9+ CD1d-autoreactive transgenic mice (24αβ), MHC-II-/-CD1d-/- mice)

dependent variables

Not explicitly mentioned

control variables

Housing conditions (naïve mice were housed in a specific pathogen-free facility)
Genetic background (Jα18-/- mice were backcrossed to B6 mice, CD1d-/- mice were backcrossed to B6 background for at least 12 generations, MR1-/- mice were backcrossed to B6 mice)

positive controls

None specified

negative controls

None specified

Annotations

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