Live/Dead Cell Viability Assay

Viability of Ca 9-22 cells was assessed by live/dead staining using the Live/Dead Cell Staining Kit (Enzo Life Sciences, Lausen, Switzerland) following the manufacturer’s guidelines [29 (link)]. Live/dead analysis was performed after seven days of culture. Cell staining was visualized using a fluorescent microscope (Nikon, Tokyo, Japan; excitation wavelength: 488 nm; emission wavelength living cells: 518 nm, green; emission wavelength dead cells: 615 nm, red).

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Nemec M., Bartholomaeus H.M., H. Bertl M., Behm C., Ali Shokoohi-Tabrizi H., Jonke E., Andrukhov O, & Rausch-Fan X. (2020). Behaviour of Human Oral Epithelial Cells Grown on Invisalign® SmartTrack® Material. Materials, 13(23), 5311.

Publication 2020

Live/Dead Cell Staining Kit

Cells Cells viability Microscope

Corresponding Organization : Medical University of Vienna

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Variable analysis

independent variables

Live/Dead Cell Staining Kit (Enzo Life Sciences, Lausen, Switzerland)

dependent variables

Viability of Ca 9-22 cells

control variables

Cell staining visualization using a fluorescent microscope (Nikon, Tokyo, Japan; excitation wavelength: 488 nm; emission wavelength living cells: 518 nm, green; emission wavelength dead cells: 615 nm, red)
Cell culture duration (seven days)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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