Quantitative RT-PCR Analysis of Rubber Tree Genes

Quantitative RT-PCR (qPCR) was performed as described previously [29 ]. The primer pairs used for the HbTPS and HbSnRK1 family genes, and SnRK1 marker genes were as listed in Table S7. The reaction was performed using the Light Cycler 2.0 system (Roche Diagnostics, Penzberg, Germany) with the SYBR Green premix kit (TaKaRa) according to the manufacturer’s instructions. The efficiency of each primer pair was evaluated and found to be between 1.844 and 1.997. For internal controls, YLS8 was used in the analysis of gene expression in the latex following the tapping treatment [65 (link)].

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Zhou B., Fang Y., Xiao X., Yang J., Qi J., Qi Q., Fan Y, & Tang C. (2022). Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree. Plants, 11(21), 2879.

Publication 2022

Light Cycler 2.0 system SYBR Green premix kit

Diagnostics Gene expression analysis Genes Latex Light Primer Rt pcr Sybr green

Corresponding Organization : Hainan University

Other organizations : Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Beijing Forestry University

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Variable analysis

independent variables

Tapping treatment

dependent variables

Expression of HbTPS and HbSnRK1 family genes
Expression of SnRK1 marker genes

control variables

YLS8 used as internal control for gene expression analysis in latex following tapping treatment

controls

Positive control: None mentioned
Negative control: None mentioned

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