Diverse Cell Lines for Cancer Research

PL-21, THP-1, MOLM-13, MV4-11, E.G7-OVA, and SEM cell lines were purchased from ATCC (USA). The E.G7-OVA cell line was modified to express full-length human EGFRvIII (Uniprot Entry P00533 AA 1-29, 298–646), resulting in E.G7-EGFRvIII cells. Luciferase-eGFP (LUC-GFP) overexpressing cell lines PL-21-LUC-GFP, THP-1-LUC-GFP and MV4-11-LUC-GFP were generated according to previously described protocols [22 (link)]. Antigen quantification of cell lines are summarized in Supplementary Table 1A. 293Vec-Galv and 293Vec-RD114 were a kind gift of Manuel Caruso, Québec, Canada and have been previously described [27 (link)]. All human cell lines were short tandem repeat profiled in house to verify their origin. Cells were used for a time period no longer than two months.