Approval for the study was granted by the Animal Research Ethics Committee of Health Sciences University Ankara Training and Research Hospital, Ankara, Turkey (meeting number: 66, decision no.: 666, dated: 26.07.2021). This experimental study was started in 2021 after the approval of the ethics committee and the study was carried out in the Animal Laboratory at Ankara Training and Research Hospital, Ankara, Turkey. The study sample comprised 21 male Wistar albino rats, each aging 8-9 weeks and weighting 250±25 grams. An environment was established before the experiment of room temperature 22-25°C, a 12-hour light/dark cycle (08:00-20:00 light; 20:00-08:00 dark), and humidity in the range of 55-60%. The rats had free access to standard rat food (23% protein, 5% fat, 15% fibre, and 50% carbohydrate) and tap water. The 21 rats were separated into 3 groups of 7. The anaesthesia procedure at the start of the study was applied to all the rats intraperitoneally with 90 mg/kg ketamine (Ketalar Flacon, 50 mg/ml Pfizer, Istanbul, Turkey) and xylazine 10 mg/kg intraperitoneal (Rompun 2% Flacon Bayer, Istanbul, Turkey). A blood sample was obtained from all the rats to determine the preoperative biochemical basal values. Laparotomy was applied to all the rats with a midline incision of approximately 3 cm.
The National Institutes of Health Guiding Principles has been taken as a guide regarding the care and use of animals used in this experimental study.12
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