Rab37 knockout (KO) mice were generated as described in Kuo's report 23 (link). CD4+ and CD8+ T cells were isolated from splenocytes derived from wild-type (WT) and Rab37 KO mice using anti-mouse CD4 magnetic particles (BD Bioscience, #551539) and mouse CD8 T lymphocyte enrichment set (BD Bioscience, # 558471) according to the manufacturer's instructions. Cells were cultured in medium containing 10% Fetal Bovine Serum (FBS, Gibco), 1% penicillin/streptomycin (Gibco), and 1% sodium pyruvate (Gibco) and incubated at 37 oC with 5% CO2 in air. Purified naïve CD4+ and CD8+ T cells were stimulated with 2 μg/mL anti-CD3/CD28 antibodies (BD Bioscience, #553057/ #553294) for 24 h or 48 h.
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