SDS-PAGE and western blot analysis were performed as previously reported [35 (link)]. The samples were probed with primary antibodies against cleaved caspase-3 (1:3000) and β-actin (1:4000), followed by HRP-conjugated anti-rabbit secondary antibodies against IgG (1:10,000). The antigens were visualized using Clarity MAXTM Western ECL Substrate (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
Free full text: Click here