C. albicans Growth Conditions

The strains of C. albicans used in this study are listed in supplemental Table S2. For routine growth, modified Lee's medium without methionine was used [31] (link), unless stated otherwise. For repression of MET3 promoter-controlled gene expression, 2.5 mM methionine and 2.5 mM cysteine were added to the medium. For GlcNAc induction, the carbon source glucose was replaced with GlcNAc (1.25% w/v) in nutrient medium. Here, agar containing Lee's medium, in which glucose was the carbon source, was referred to as glucose agar and Lee's medium containing GlcNAc as a carbon source was referred to as GlcNAc agar. Agar cultures were grown at a density of 80–120 colonies per 85 mm plate. Phloxine B was added to nutrient agar for opaque colony staining [46] (link).