ATPase activity determination was carried out using ABCB1 PREDEASY ATPase kit and Pgp-Glo Assay System kit according to manufacturer's instructions (Promega and Solvo Biotechnology, respectively). Rho-123 accumulation assay was carried out as described previously [71 (link)]. Determination of intracellular ATP content was performed using EnzyLight ATP Assay kit according to manufacturer's instructions (BioAssay Systems).
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