cDNA was generated using SuperScript cDNA Kit (Invitrogen). qRT-PCR was performed in PCR System with SYBR Green (Roche Diagnostics, Burgess Hill, UK). GADPH or U6 was used as the internal reference. circSMAD2, miR-1277-5p, and MFGE8 relative expression was measured using the 2-ΔΔCt method. Primer sequences are listed in Table S1 .
Protocol detail
Quantifying circSMAD2, miR-1277-5p, and MFGE8
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Cdna
Diagnostics
Mfge8
Primer
Sybr green
Corresponding Organization :
Other organizations : Shanxi Medical University
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Variable analysis
independent variables
- CDNA generation using SuperScript cDNA Kit (Invitrogen)
- CircSMAD2 relative expression
- MiR-1277-5p relative expression
- MFGE8 relative expression
- GADPH or U6 as the internal reference
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