RNA-FISH was performed according to the protocol of the RNAscope Multiplex Fluorescent v2 Assay (cat: 323110, Acdbio), which detects single mRNA molecules. In briefly, E9.5 embryos were fixed for 20–30 h in 4% paraformaldehyde and then dehydrated in methanol. The following probes were used: mm-Sox10-C2 (cat: 43591-C2, Acdbio), mm-Robo2-C1 (cat: 475961, Acdbio). Whole-mount and sections RNA-FISH were performed as previously described73 (link). Embryos were imaged using an AxioZoom.V16 microscope (Zeiss) with ×40 objective and photographed with an Axiocam digital camera (Zen 2011, Zeiss). Sections were examined using an Apotome (Zeiss), 10X objective and photographed with an Axiocam digital camera (Zen 2011, Zeiss).
Odelin G., Faucherre A., Marchese D., Pinard A., Jaouadi H., Le Scouarnec S., Chiarelli R., Achouri Y., Faure E., Herbane M., Théron A., Avierinos J.F., Jopling C., Collod-Béroud G., Rezsohazy R, & Zaffran S. (2023). Variations in the poly-histidine repeat motif of HOXA1 contribute to bicuspid aortic valve in mouse and zebrafish. Nature Communications, 14, 1543.
Assay Camera Embryos FishMethanol Microscope Mrna Paraformaldehyde Sox10
Corresponding Organization :
Other organizations :
Aix-Marseille Université, Inserm, Université de Montpellier, Centre National de la Recherche Scientifique, UCLouvain, Institut du Thorax, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Centre National de Recherche en Génomique Humaine, Fondation Jean Dausset-CEPH, Université de Bretagne Occidentale, École nationale d'ingénieurs de Brest, de Duve Institute, Hôpital de la Timone
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