Co-immunoprecipitation assay was performed as previously described (Sun et al., 2018 (link)). Cells were lysed in immunoprecipitation buffer supplemented with complete protease inhibitor. After lysing with ultrasound, lysates were centrifuged to remove insoluble components. Lysates were then incubated overnight with rabbit anti-E-cadherin (Cell Signaling Technology) or rabbit IgG primary antibodies at 4°C. Protein G beads (Beyotime) were then added to the lysates. The precipitated proteins were measured via western blot analysis as described above.
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