Harzianic Acid Purification from Trichoderma

Harzianic acid was obtained from T. harzianum M10 grown in liquid medium (PDB, HiMedia, Mumbai, India) for 21 days and purified following a previously described protocol with some modifications [25 (link)]. Briefly, culture filtrate was exhaustively extracted with ethyl acetate (EtOAc, Carlo Erba, Cornaredo, Milan, Italy), and the dry residue was resuspended in dichloromethane (DCM, Carlo Erba) and extracted with a 2M solution of sodium hydroxide (NaOH, Carlo Erba). The aqueous phase was acidified at pH = 2 with hydrochloric acid (HCl, Carlo Erba), and harzianic acid was obtained after vacuum filtration and precipitate wash with EtOAc. Harzianic acid identification was achieved by NMR and LC-MS analyses [30 (link),31 (link)].

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Staropoli A., Cuomo P., Salvatore M.M., De Tommaso G., Iuliano M., Andolfi A., Tenore G.C., Capparelli R, & Vinale F. (2023). Harzianic Acid Activity against Staphylococcus aureus and Its Role in Calcium Regulation. Toxins, 15(4), 237.

Publication 2023

PDB EtOAc NaOH HCl

Dichloromethane Erba Ethyl acetate Filtration Harzianic acid Hydrochloric acid Sodium hydroxide Vacuum

Corresponding Organization : Federico II University Hospital

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Variable analysis

independent variables

Growth medium (PDB)
Incubation time (21 days)
Extraction solvent (ethyl acetate, dichloromethane)

dependent variables

Harzianic acid production and purification

control variables

Temperature (not specified)
PH (adjusted to 2 for precipitation)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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