Multimodal Stimulation of Cochlear Responses

Stimuli were generated via a custom-made system based on NI-DAQ-Cards (NI PCI-6229; National Instruments; Austin, USA) controlled with custom-written MATLAB scripts (The MathWorks, Inc.; Natick, USA). Acoustic stimuli (sampling rate = 830 kHz) were presented open-field via a loudspeaker (Avisoft Inc., Germany) localized on the left side at ∼15 cm from the pinna. A 0.25-inch microphone and measurement amplifier (D4039; 2610; Brüel & Kjaer GmbH, Naerum, Denmark) were used to calibrate sound pressure levels. Optical stimuli were delivered at the cochlear round window via an optical fiber (200 μm diameter, 0.39 NA, Thorlabs GmbH, Germany) coupled to a blue laser (473 nm, MLLFN-473-100, 100 mW diode pumped solid state [DPSS]; Changchun New Industry Optoelectronics). The maximum light intensity (or radiant flux) was measured before every experiment (LaserCheck; Coherent Inc.) and later used for calibration. The round window was exposed as previously described [[13] , [14] , [15] (link),72 ]. Briefly, the round window was exposed by opening the temporal bone ventrally from the stylomastoid foramen and the exact location of the round window was determined by visually following the stapedial artery.

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Mittring A., Moser T, & Huet A.T. (2023). Graded optogenetic activation of the auditory pathway for hearing restoration. Brain Stimulation, 16(2), 466-483.

Publication 2023

MATLAB scripts LaserCheck

Acoustic Artery Austin Light Optical Pinna Pressure Round window Sound Stylomastoid foramen Temporal bone

Corresponding Organization :

Other organizations : University of Göttingen, German Primate Center

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Variable analysis

independent variables

Acoustic stimuli (sampling rate = 830 kHz) presented open-field via a loudspeaker (Avisoft Inc., Germany) localized on the left side at ∼15 cm from the pinna
Optical stimuli delivered at the cochlear round window via an optical fiber (200 μm diameter, 0.39 NA, Thorlabs GmbH, Germany) coupled to a blue laser (473 nm, MLLFN-473-100, 100 mW diode pumped solid state [DPSS]; Changchun New Industry Optoelectronics)

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Sound pressure levels calibrated using a 0.25-inch microphone and measurement amplifier (D4039; 2610; Brüel & Kjaer GmbH, Naerum, Denmark)
Light intensity (or radiant flux) measured before every experiment (LaserCheck; Coherent Inc.) and later used for calibration
Round window exposure by opening the temporal bone ventrally from the stylomastoid foramen and determining the exact location of the round window by visually following the stapedial artery

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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