Purification of Recombinant LukS-PV Toxin

The LukS-PV sequence was amplified from PVL-positive Staphylococcus aureus isolates by polymerase chain reaction (PCR) using the following primers: LukS-FW, 5ʹ-acgcGGATCCGAATCTAAAGCTGATAACAATATTGAGAATATTG-3ʹ; LukS-RV, 5ʹ-accgCTCGAGTCAATTATGTCCTTTCACTTTAATTTCATGAG-3ʹ. Recombinant LukS-PV was generated as described previously [27 (link)]. Purification of recombinant LukS-PV was performed with the His-Bind® Purification Kit (Millipore, USA) in accordance with the manufacturer's instructions.

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Xu X., Ding P., Shi L., Wu G, & Ma X. (2022). LukS-PV inhibits hepatocellular carcinoma cells migration by downregulating HDAC6 expression. BMC Cancer, 22, 630.

Publication 2022

His-Bind® Purification Kit

Polymerase chain reaction Primers Staphylococcus aureus

Corresponding Organization :

Other organizations : University of Science and Technology of China

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Variable analysis

independent variables

LukS-PV sequence amplification from PVL-positive Staphylococcus aureus isolates by polymerase chain reaction (PCR) using the following primers: LukS-FW, 5ʹ-acgcGGATCCGAATCTAAAGCTGATAACAATATTGAGAATATTG-3ʹ; LukS-RV, 5ʹ-accgCTCGAGTCAATTATGTCCTTTCACTTTAATTTCATGAG-3ʹ

dependent variables

Purification of recombinant LukS-PV using the His-Bind® Purification Kit (Millipore, USA)

control variables

Manufacturer's instructions for the His-Bind® Purification Kit (Millipore, USA)

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