Quantifying THP-1 Cell Adhesion to RASFs

RASFs were incubated with various concentrations of Antcin K for 24 h at 37°C and then with BCECF-AM-labeled THP-1 cells for 1 h at 37°C. Non-adherent cells were removed by PBS washing. Attached THP-1 cells in RASFs were counted using a Nikon Eclipse Ti-S Fluorescent microscope (Nikon, Japan) with images captured at 10× magnification. Captured images were counted and quantified by MacBiophotonics ImageJ software (v1.51, National Institutes of Health, Bethesda, MD, USA) (19 (link), 20 (link), 27 (link)).

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Achudhan D., Li-Yun Chang S., Liu S.C., Lin Y.Y., Huang W.C., Wu Y.C., Huang C.C., Tsai C.H., Ko C.Y., Kuo Y.H, & Tang C.H. (2022). Antcin K inhibits VCAM-1-dependent monocyte adhesion in human rheumatoid arthritis synovial fibroblasts. Food & Nutrition Research, 66, 10.29219/fnr.v66.8645.

Publication 2022

Nikon Eclipse Ti-S Fluorescent microscope

Antcin k Bcecf am Cells Microscope Thp 1 cells

Corresponding Organization :

Other organizations : China Medical University, China Medical University Beigang Hospital, Asia University, China Medical University Hospital

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Variable analysis

independent variables

Antcin K concentrations

dependent variables

Attached THP-1 cells in RASFs

control variables

Incubation time (24 h)
Incubation temperature (37°C)
BCECF-AM-labeled THP-1 cells incubation time (1 h)
Imaging magnification (10×)

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