Biochemical Assays for Ocular Biomarkers

The level of ATX in AH was determined using a two-site immunoenzymetric assay with an ATX assay reagent by means of a Tosoh AIA system (Tosoh, Tokyo, Japan), as described previously [11 (link), 12 (link), 18 (link)]. TGF-β levels in AH were measured using the Bio-Plex Pro TGF-β Assay (Bio-Rad, Hercules, CA, USA), in accordance with the manufacturer’s protocol. LysoPLD activity in the culture medium was determined as previously described [11 (link), 12 (link), 18 (link)].

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Igarashi N., Honjo M., Yamagishi R., Kurano M., Yatomi Y., Igarashi K., Kaburaki T, & Aihara M. (2021). Crosstalk between transforming growth factor β-2 and Autotaxin in trabecular meshwork and different subtypes of glaucoma. Journal of Biomedical Science, 28, 47.

Publication 2021

AIA system Bio-Plex Pro TGF-β Assay

Assay Culture medium Tgf β

Corresponding Organization :

Other organizations : The University of Tokyo, Tosoh (Japan)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

ATX levels in AH
TGF-β levels in AH
LysoPLD activity in the culture medium

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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