Western Blot Analysis of Protein Expression

Western blot analyses were performed using a custom-made rabbit anti-TRIP6 monoclonal antibody^{16 (link)}, a rabbit anti-mCherry antibody (ab167453, abcam, Cambridge, UK), a rabbit anti-MYOG antibody (ab124800, abcam), a Goat anti-TNNI2 antibody (EB12036, Everest Biotech, Upper Heyford, UK), a mouse anti-beta-actin (AC-15, Sigma-Aldrich) and a rabbit anti-glucocorticoid receptor (GR) antibody (sc1002, Santa Cruz, Heidelberg, Germany) which was used as a loading control. Signals were detected by enhanced chemoluminescence using the ChemiDoc Touch Imaging System (BioRad laboratories, Munich, Germany). Signal quantification was performed within the linear range of detection using the Image Lab software (Bio-Rad laboratories). Linear brightness and contrast adjustments were made for illustration purposes only after the analysis had been made.

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Norizadeh Abbariki T., Gonda Z., Kemler D., Urbanek P., Wagner T., Litfin M., Wang Z.Q., Herrlich P, & Kassel O. (2021). The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation. Scientific Reports, 11, 12904.

Publication 2021

ab167453 ab124800 AC-15 sc1002 ChemiDoc Touch Imaging System Image Lab software

Anti antibody Beta actin Glucocorticoid receptor Goat Mouse Rabbit Touch Western blot analyses

Corresponding Organization :

Other organizations : Karlsruhe Institute of Technology, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)

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Variable analysis

independent variables

Custom-made rabbit anti-TRIP6 monoclonal antibody
Rabbit anti-mCherry antibody
Rabbit anti-MYOG antibody
Goat anti-TNNI2 antibody
Mouse anti-beta-actin
Rabbit anti-glucocorticoid receptor (GR) antibody

dependent variables

Protein expression levels detected by Western blot analysis

control variables

Rabbit anti-glucocorticoid receptor (GR) antibody used as a loading control

controls

Positive control: Not specified
Negative control: Not specified

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