Profiling miR-150-5p and miR-150-3p

A549 cells were transfected with 10 nM miRNAs by reverse transfection. After 72 h, immunoprecipitation was performed using a microRNA Isolation Kit, Human Ago2 (Wako Pure Chemical Industries, Ltd., Osaka, Japan) as described previously [47 (link),48 (link)]. Expression levels of miR-150-5p and miR-150-3p were analyzed by qRT-PCR. MiRNA data were normalized to expressions of miR-16-5p, miR-21-5p, and miR-26a, which were not affected by miR-150-5p and miR-150-3p.

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Misono S., Seki N., Mizuno K., Yamada Y., Uchida A., Sanada H., Moriya S., Kikkawa N., Kumamoto T., Suetsugu T, & Inoue H. (2019). Molecular Pathogenesis of Gene Regulation by the miR-150 Duplex: miR-150-3p Regulates TNS4 in Lung Adenocarcinoma. Cancers, 11(5), 601.

Publication 2019

Human Ago2

A549 cells Ago2 Human Immunoprecipitation Isolation Mirna Transfection

Corresponding Organization : Chiba University

Other organizations : Kagoshima University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

10 nM miRNAs

dependent variables

Expression levels of miR-150-5p and miR-150-3p

control variables

MiR-16-5p
MiR-21-5p
MiR-26a

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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