Total RNA (100 ng) was reverse-transcribed to cDNA using a High Capacity cDNA Archive Kit (LIFE TECHNOLOGIES; Carlsbad, CA, USA). TaqMan PCR was carried out using the TaqMan Fast Advanced PCR master mix and TaqMan gene expression assays (all reagents from LIFE TECHNOLOGIES), by means of a 7900HT Fast Real-Time PCR System (APPLIED BIOSYSTEMS) as previously reported40 (link).
Free full text: Click here