Radiosynthesis and Characterization of [18F]AlF-mNOTA-GZP

The synthesis of NOTA–β-Ala-Gly-Gly-Ile-Glu-Phe-Asp-CHO (mNOTA-GZP) and characterisation details have been described previously [16 (link)]. The radiosynthesis of [¹⁸F]AlF-mNOTA-GZP has also been described previously [16 (link)]. No-carrier-added aqueous [¹⁸F]fluoride ion was produced via the [¹⁸O(p,n)¹⁸F] nuclear reaction (GE PETtrace 860 cyclotron). Quality control analytical radio-HPLC was performed on a UFLC Shimazdu HPLC system equipped with dual-wavelength UV detector and a NaI/PMT-radiodetector (Flow-Ram, LabLogic). Radioactivity measurements were made with a CRC-55tPET dose calibrator (Capintec, USA). [¹⁸F]AlF-mNOTA-GZP was formulated as a colourless solution of 10% ethanol in saline (pH = 7.4) with a nondecay corrected radiochemical yield of 12-18%, a radiochemical purity of 99%, and molar activity of 50-81 GBq/μmol (n = 3) after a total reaction and purification time of 50 minutes.

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Goggi J.L., Ramasamy B., Tan Y.X., Hartimath S.V., Tang J.R., Cheng P., Msallam R., Chacko A.M., Hwang Y.Y, & Robins E.G. (2021). Granzyme B PET Imaging Stratifies Immune Checkpoint Inhibitor Response in Hepatocellular Carcinoma. Molecular Imaging, 2021, 9305277.

Publication 2021

PETtrace 860 cyclotron CRC-55tPET dose calibrator

Ala gly gly Cyclotron Ethanol Fluoride ion Glu asp Hplc Molar Nota Radioactivity Radiochemical Saline Synthesis

Corresponding Organization : Agency for Science, Technology and Research

Other organizations : Duke-NUS Medical School, Singapore Immunology Network, National University of Singapore

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Variable analysis

independent variables

No-carrier-added aqueous [18F]fluoride ion produced via the [18O(p,n)18F] nuclear reaction

dependent variables

Radiochemical yield of [18F]AlF-mNOTA-GZP
Radiochemical purity of [18F]AlF-mNOTA-GZP
Molar activity of [18F]AlF-mNOTA-GZP

control variables

Total reaction and purification time of 50 minutes
Formulation of [18F]AlF-mNOTA-GZP as a colourless solution of 10% ethanol in saline (pH = 7.4)

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