The animal was deeply anesthetized with isoflurane and the first eye was enucleated. After the enucleation of the second eye, the animal was euthanized by CO2 inhalation. The retinal flatmounts were prepared as previously described (10 (link)). Briefly, an incision was made along the corneal limbus, the lens and sclera were removed, and radial cuts were made to relieve the curvature. The flat-mounted retina was transferred to a glass bottom dish (MatTek Corp.) and incubated at room temperature in the Ames’ medium (A1420, Sigma-Aldrich) oxygenated with 95%O2/5%CO2.
Protocol detail
Retinal Flatmount Preparation
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Corresponding Organization : Indiana University Bloomington
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Variable analysis
independent variables
- Enucleation of the first eye
- Enucleation of the second eye
- CO2 inhalation for euthanasia
- Retinal flatmounts prepared as previously described (10)
- Anesthesia with isoflurane
- Incubation of the flat-mounted retina in Ames' medium oxygenated with 95%O2/5%CO2 at room temperature
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