Levels of NAA were measured in postmortem MS and control brain tissue and in brains of WT and NAT8L ± mice by high-performance liquid chromatography (HPLC) as previously described (Li et al. 2013 (link)). NAA was measured in 50 mg cortical gray matter tissue adjacent to the NAWM analyzed for myelin lipid composition by mass spectrometry. A Whatman partisil 10 SAX anion-exchange column (4.6 mm × 250 mm) was used in an Agilent 1100 Series HPLC Value System. NAA concentrations were determined in triplicate. Pearson’s correlation analyses were performed to identify lipid changes in postmortem samples that were correlated with changes in NAA concentration. Correlations were considered significant if p < 0.05.
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N-Acetylaspartate Measurement in MS
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Anion
Brains
Cortical
Gray matter
High performance liquid chromatography
Lipid
Mass spectrometry
Mice
Myelin
Postmortem
Postmortem changes
Tissue
Corresponding Organization :
Other organizations : Kent State University, University of Akron, University of California, Davis
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Variable analysis
independent variables
- Genotype (WT and NAT8L ± mice)
- Levels of NAA measured in postmortem MS and control brain tissue and in brains of WT and NAT8L ± mice
- 50 mg cortical gray matter tissue adjacent to the NAWM analyzed for myelin lipid composition by mass spectrometry
- Whatman partisil 10 SAX anion-exchange column (4.6 mm × 250 mm) used in an Agilent 1100 Series HPLC Value System
- Positive control: Postmortem control brain tissue
- Negative control: Wildtype (WT) mice
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