Bilateral fibular fractures were conducted using previously described standard protocols[33 (link),34 (link)]. In brief, under isoflurane anaesthesia, a 5 mm skin incision was made over the fibula. Using microtenotomy scissors, mice received a bilateral transverse and non-comminuted fibular fracture at the midpoint of the fibula approximately 12 mm proximal to the calcaneal tuberosity. Skin incisions were closed using skin glue (3M™ Vetbond™ Tissue Adhesive, St. Paul, MN, USA). Immediately post-fracture, a 10 mm incision was made on the dorsal surface of mice in between the scapulae. Mini-osmotic pumps (ALZET® Model 1002, DURECT Corporation, CA, USA) were then subcutaneously inserted and incisions were closed using Reflex wound clips.
Protocol detail
Bilateral fibular fracture model
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Anaesthesia
Calcaneal
Clips
Fibula
Fracture
Fracture of the fibular
Isoflurane
Mice
Osmotic
Reflex
Scapulae
Skin
Tissue adhesive
Wound
Corresponding Organization :
Other organizations : La Trobe University, Garvan Institute of Medical Research
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Variable analysis
independent variables
- Bilateral transverse and non-comminuted fibular fracture at the midpoint of the fibula approximately 12 mm proximal to the calcaneal tuberosity
- Not explicitly mentioned
- Isoflurane anaesthesia
- Skin incision location (5 mm over the fibula)
- Skin incision closure using skin glue
- Subcutaneous insertion of mini-osmotic pumps
- Reflex wound clips to close incisions
- No positive or negative controls were explicitly mentioned in the provided information.
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