Fatty Acid Composition Analysis by GC/MS

Fatty acid composition was analyzed by gas chromatography/mass spectrometry (GC/MS) using the fatty acid methyl ester (FAME) method [51 (link)]. For each sample, roughly 400 worms were collected in water, allowed to settle, and most of the water removed before freezing the worm pellet. 2.5% sulfuric acid in methanol was added to frozen pellets and then incubated at 70°C for one hour in a glass tube. FAMEs were extracted in hexane and separated using an Agilent 7890 GC/5975C MS in scanning ion mode equipped with a SP-1380 column. Relative amounts of fatty acid methyl esters are reported.

Free full text: Click here

Perez M.A., Clostio A.J., Houston I.R., Ruiz J., Magtanong L., Dixon S.J, & Watts J.L. (2022). Ether lipid deficiency disrupts lipid homeostasis leading to ferroptosis sensitivity. PLoS Genetics, 18(9), e1010436.

Publication 2022

7890 GC/5975C MS

Esters Fatty acid Frozen Gc ms Hexane Methanol Pellets Sulfuric acid Worm

Corresponding Organization : Stanford University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative amounts of fatty acid methyl esters

control variables

Sample size (roughly 400 worms collected)
Sample preparation (allowed to settle, most water removed, frozen pellet)
Fatty acid extraction (2.5% sulfuric acid in methanol, incubation at 70°C for 1 hour)
FAME separation (extracted in hexane, separated using Agilent 7890 GC/5975C MS with SP-1380 column)

controls

No positive or negative controls explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!