The effects of the three plant extracts on cell viability were evaluated on human epithelial colorectal adenocarcinoma cells (Caco-2 cells). Caco-2 cells are a valid system for evaluating the biological effects of plant extracts for human use [57 (link),58 (link),59 (link)]. Caco-2 cells were obtained from the ATCC cell bank (Rockville, MD, USA) and were propagated in DMEM (Gibco BRL, Life Technologies, Mumbai, India) supplemented with 10% fetal calf serum, 1% sodium pyruvate, 1% L-glutamine solution, and 1% streptomycin/penicillin in a humidified atmosphere of 5% CO2 at 37 °C.
The effect of Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. flower extracts on cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to obtain the range of toxic and nontoxic concentrations. Caco-2 cells were seeded in 96-well plates at a density of 5 × 104 cells per well for 24 h in complete medium. Cells were then treated with flower extracts at various concentrations (1–50 μg/mL) or with vehicle (DMSO at 0.001–0.05%) for 48 h; then, the cells were exposed to the MTT reagent (0.4 mg/mL in PBS) for 30 min at 37 °C, 5% CO2. The absorbance at 570 nm was measured using the microplate reader MPR A4i (Tosoh, Tokyo, Japan).
The effect of Artocarpus lakoocha Roxb. and Artocarpus heterophyllus Lam. flower extracts on cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to obtain the range of toxic and nontoxic concentrations. Caco-2 cells were seeded in 96-well plates at a density of 5 × 104 cells per well for 24 h in complete medium. Cells were then treated with flower extracts at various concentrations (1–50 μg/mL) or with vehicle (DMSO at 0.001–0.05%) for 48 h; then, the cells were exposed to the MTT reagent (0.4 mg/mL in PBS) for 30 min at 37 °C, 5% CO2. The absorbance at 570 nm was measured using the microplate reader MPR A4i (Tosoh, Tokyo, Japan).
Free full text: Click here
