We used a common variant, rs9939609 (A/T) repeatedly identified in many BMI GWAS in EA and AA 27 (link)–29 (link). Given the lack of association of the rs9939609 SNP in some studies of AA 30 (link), we also considered other SNPs identified in fine-mapping efforts in AA: rs1421085, rs56137030, rs17817964, and rs8050136 31 (link),32 (link). Genotyping was originally performed using the Affymetrix 500 SNP Array in FHS, and the Affymetrix 6.0 SNP Array in WHI (Affymetrix Inc, Santa Clara, CA). In WHI the DNA was extracted from blood samples collected at enrollment, and genotyping QC included concordance rates for blinded and unblended duplicates 33 (link). In order to test multiple FTO SNPs in WHI, we used imputed genotypes which were available in dbGaP. Briefly, genotypes were imputed using BEAGLE software 34 (link), and 1,000 Genomes data 35 (link) as reference. Individuals with missing values of FTO genotypes were excluded from all analyses.
Protocol detail
FTO Genetic Variants and BMI Associations
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Arizona
Top 5 similar protocols
Variable analysis
independent variables
- Rs9939609 (A/T)
- Rs1421085
- Rs56137030
- Rs17817964
- Rs8050136
- Body Mass Index (BMI)
- Genotyping quality control, including concordance rates for blinded and unblended duplicates
- Imputation of genotypes using BEAGLE software and 1,000 Genomes data as reference
- Exclusion of individuals with missing values of FTO genotypes
- Positive controls not explicitly mentioned.
- Negative controls not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!