Quantifying Jasmonic and Salicylic Acid

To measure the endogenous concentrations of JA and SA, root samples of about 100–200 mg were homogenised twice with 80% (V/V) cold methanol and shaken overnight in 4°C darkness. Dissolution, filtration, storage and quantification of combined extracts are described by Sun et al. (2014) (link). SA and JA content were measured by HPLC-MS/MS performed by AB SCIEX QTRAP 4500 system (AB SCIEX, Foster City, CA, United States) as described by Zhou et al. (2018) (link). The experiment was repeated 3 times with more than 3 seedlings in the sample.

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Wei T., Tang Y., Jia P., Zeng Y., Wang B., Wu P., Quan Y., Chen A., Li Y, & Wu J. (2021). A Cotton Lignin Biosynthesis Gene, GhLAC4, Fine-Tuned by ghr-miR397 Modulates Plant Resistance Against Verticillium dahliae. Frontiers in Plant Science, 12, 743795.

Publication 2021

QTRAP 4500 system

Cold Darkness Filtration Hplc Methanol Ms ms Root Seedlings

Corresponding Organization : Chinese Academy of Sciences

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Endogenous concentrations of jasmonic acid (JA)
Endogenous concentrations of salicylic acid (SA)

control variables

Root sample weight (100-200 mg)
Homogenization method (twice with 80% (V/V) cold methanol)
Extraction duration (shaken overnight in 4°C darkness)
Quantification method (HPLC-MS/MS performed by AB SCIEX QTRAP 4500 system)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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