Isolation and Cultivation of Marine Bacteria

Streptomyces mobaraensis DSM 40847 was purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). The marine bacteria V. alginolyticus B1, Erythrobacter sp. HKB8, Ruegeria B32, S. aureus B04, S. hominis N32, S. arlettae OM, M. esteraromaticum N22, I. sediminum N28, Pseudoalteromonas L001, and E. coli N57 were isolated from marine biofilms and obtained from the culture collection of our laboratory [24 (link)]. Soybean powder was purchased from Wugumf, Shenzhen, China. Soluble starch was purchased from Affymetrix, Santa Clara, CA, USA. Magnesium sulfate hydrate was purchased from Riedel-de-Haën, Seelze, Germany. Bacteriological peptone was obtained from Oxoid, Milan, Italy. Mueller-Hinton broth (MHB) was purchased from Fluka Chemie AG, Buchs, Switzerland. Phosphate-buffered saline (PBS) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Thermo Fisher Scientific Inc., San Jose, CA, USA. Lysogeny broth (LB), glucose, and 1-butanol were purchased from VWR International Ltd., Leicestershire, UK. All other chemicals were supplied by Sigma-Aldrich Corporation, Saint Louis, MO, USA.