Streptozotocin-Induced Type 1 Diabetes Mouse Model

In order to establish the type 1 diabetes mouse models, the mice were subjected to overnight fasting, following which they were administered intraperitoneal (i.p.) injections of freshly prepared streptozotocin (STZ, 50 mg/kg/day, Sigma 0130) for five consecutive days. The control group mice were administered citrate buffer (5 mL/kg/day) for 5 days [22 (link)]. Fasting blood glucose levels were measured from the tail vein using the ACCU-CHEK^® Mobile blood glucose meter (Roche). If the fasting blood glucose level was above 11.1 mmol/L, the mouse was considered diabetic and used for subsequent analyses. The body weights of the mice were also recorded.