Colon Inflammation Biomarker Detection

The tissue samples of distal colon were collected from the inferior vena cava. After centrifugation, the sample was collected for the detection of inflammatory factors as previous report [9 (link)]. Total RNA was extracted according to RNA extraction kit (ER501-01, TransGen Biotech, China). Real-time quantitative PCR (RT-qPCR) was carried out according to SYBR Green Real-Time PCR MasterMix (AQ602-14, TransGen Biotech, China). The expression level of β-actin was used as reference. All primer sequences used in this study are given in Table 1.

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Huang H., Zhao P., Xi M., Li F, & Ji L. (2021). Mechanism of QingHuaZhiXie Prescription Regulating TLR4-IECs Pathway in the Intervention of Diarrhea Predominant Irritable Bowel Syndrome. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 5792130.

Publication 2021

RNA extraction kit SYBR Green Real-Time PCR MasterMix

Actin Centrifugation Colon Inferior vena cava Inflammatory Primer Real time pcr Sybr green Tissue

Corresponding Organization : Nanjing University of Chinese Medicine

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Expression level of β-actin
Expression levels of inflammatory factors (as per previous report [9])

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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