Ultrapure water (18.2 MΩ
cm, ELGA Water purification system, Purelab Ultra MK 2, UK) was used
for all dilutions and washing steps. A multi-element stock solution
and single element standard solutions were purchased from Labkings
(Hilversum, The Netherlands). Bovine serum albumin (lyophilized powder,
BioReagent), Tris buffered saline (BioUltra), Triton X-100 (for molecular
biology), m-xylene (anhydrous, ≥99%), and ethanol (absolute,
EMSURE) were purchased from Sigma-Aldrich (Steinheim, Germany). The
target retrieval solution was bought from Agilent Technologies (Waldbronn,
Germany). Paraformaldehyde aqueous solution (Electron Microscopy grade,
16%) was obtained from Science Services (Munich, Germany) in form
of sealed ampoules, to ensure fresh solutions in each staining procedure.
The metal-conjugated antibodies used in this study (Table S3 ) and the Intercalator-Ir (Cell-ID, 125 μM)
were purchased from Fluidigm (San Francisco, CA, USA). LA-ICP-TOFMS
measurements were carried out in an ISO class 7 clean room. All cell
culture media and reagents were purchased from Sigma-Aldrich (Vienna,
Austria) and all plastic dishes, plates, and flasks from StarLab (Hamburg,
Germany) unless stated otherwise.
cm, ELGA Water purification system, Purelab Ultra MK 2, UK) was used
for all dilutions and washing steps. A multi-element stock solution
and single element standard solutions were purchased from Labkings
(Hilversum, The Netherlands). Bovine serum albumin (lyophilized powder,
BioReagent), Tris buffered saline (BioUltra), Triton X-100 (for molecular
biology), m-xylene (anhydrous, ≥99%), and ethanol (absolute,
EMSURE) were purchased from Sigma-Aldrich (Steinheim, Germany). The
target retrieval solution was bought from Agilent Technologies (Waldbronn,
Germany). Paraformaldehyde aqueous solution (Electron Microscopy grade,
16%) was obtained from Science Services (Munich, Germany) in form
of sealed ampoules, to ensure fresh solutions in each staining procedure.
The metal-conjugated antibodies used in this study (
were purchased from Fluidigm (San Francisco, CA, USA). LA-ICP-TOFMS
measurements were carried out in an ISO class 7 clean room. All cell
culture media and reagents were purchased from Sigma-Aldrich (Vienna,
Austria) and all plastic dishes, plates, and flasks from StarLab (Hamburg,
Germany) unless stated otherwise.
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