Optogenetic Manipulation of DRN Neurons

Experiments were performed on male and female C57/Bl6 mice aged 4–8 weeks. Slc6a4-cre::Rosa-TdTomato (SERT-Cre) and Sst-cre::Rosa-TdTomato transgenic lines were used to fluorescently label DRN 5-HT and somatostatin (SOM) GABA neurons, respectively. Animals were group-housed and kept on a 12:12 hr light/dark cycle with access to food and water ad libitum. All experiments were carried out in accordance with procedures approved by the University of Ottawa Animal Care and Veterinary Services (protocol numbers CMM-164, CMM-176, CMM-1711, CMM-1743, and CMM-2737).

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Harkin E.F., Lynn M.B., Payeur A., Boucher J.F., Caya-Bissonnette L., Cyr D., Stewart C., Longtin A., Naud R, & Béïque J.C. (2023). Temporal derivative computation in the dorsal raphe network revealed by an experimentally driven augmented integrate-and-fire modeling framework. eLife, 12, e72951.

Publication 2023

Animal Female Food Gaba neurons Male Mice Rosa Slc6a4 Somatostatin Tdtomato Transgenic

Corresponding Organization :

Other organizations : University of Ottawa, MIND Research Institute

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Variable analysis

independent variables

Genotype (Slc6a4-cre::Rosa-TdTomato (SERT-Cre) and Sst-cre::Rosa-TdTomato transgenic lines)

dependent variables

Fluorescent labeling of DRN 5-HT and somatostatin (SOM) GABA neurons

control variables

Sex (male and female C57/Bl6 mice)
Age (4–8 weeks)
Housing (group-housed)
Light/dark cycle (12:12 hr)
Food and water access (ad libitum)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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