Diverse Melanoma Cell Lines for Research

Human primary uveal melanoma cell lines (92.1, Mel270, and Mel285), human uveal melanoma liver metastasis cell lines (OMM2.3 and MM28), primary cutaneous melanoma cell lines (OCM3 and A375), and cutaneous melanoma lymph node metastasis cell lines (Hs294t and Sk‐Mel28) were studied. Cell lines mutational profile and original references are summarized on Table S1. OMM2.3, Mel 270, and Mel 285 cell lines were kindly provided by Dr Bruce R. Ksander (Schepens Eye Research Institute, Boston, MA, USA). MM28 and OCM3 cell lines were kindly provided by Dr Martine J. Jager (Leiden University Medical Center, Leiden, the Netherlands). SK‐Mel28, Hs294t, and A375 cell lines were purchased from ATCC (#HTB‐72, #HTB‐140, #CRL‐1619, ATCC, Manassas, VA, USA). Cell line 92.1 was purchased from Millipore Sigma (#13012458‐1VL, Millipore Sigma, Burlington, MA, USA). Cells were cultured in a 37 °C humidified incubator under 5% CO₂ atmosphere. Mel285 and Mel270 were cultured in RPMI 1640 medium (#11875093, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% nonessential amino acids (#11140050, Thermo Fisher Scientific), 1% vitamin solution (#11120052, Thermo Fisher Scientific), 100 U·mL⁻¹ penicillin, 100 U·mL⁻¹ streptomycin (#15140122, Thermo Fisher Scientific), and 10% heat‐inactivated FBS (#10438026, Thermo Fisher Scientific). 92.1, OCM3, OMM2.3, and THP‐1 were cultured in RPMI 1640 medium supplemented with 100 U·mL⁻¹ penicillin, 100 U·mL⁻¹ streptomycin, and 10% heat‐inactivated FBS. MM28 was cultured in IMDM (#12440053, Thermo Fisher) supplemented with 200 U·mL⁻¹ penicillin, 200 U·mL⁻¹ streptomycin, and 20% heat‐inactivated FBS. Hs294t and A375 were cultured in DMEM medium (#11965092, Thermo Fisher Scientific) supplemented with 100 U·mL⁻¹ penicillin, 100 U·mL⁻¹ streptomycin, and 10% FBS. Sk‐Mel28 was cultured in EMEM medium (#30‐2003, ATCC) supplemented with 100 U·mL⁻¹ penicillin, 100 U·mL⁻¹ streptomycin, and 10% FBS. Cells were passaged by trypsinization with 0.25% EDTA trypsin (#25200056, Thermo Fisher Scientific) when they reached 90% confluency using sterile technique. Medium was changed every 2–3 days.