Comparing Mosquito Biting and Landing

Two FTPE were positioned on the ground at 2.5-m distance either side of the volunteer and at 2 m from the back of the chamber (Fig. 3). Fifty pyrethroid-susceptible Ae. aegypti mosquitoes (Bagamoyo strain) were then released into the chamber (Fig. 2). A total of three replicates for the biting experiment and three for the landing method were conducted over 3 consecutive days, between 0630 and 0730 hours for the former and between 0830 and 0930 hours for the latter. This order was switched for the remaining 3 experimental days, with the landing method conducted first to control for temporal bias when comparing the results of the two methods, which could have been affected by temperature and mosquito circadian rhythm.