The leukocyte homing assay was performed as described previously (4 (link)). Peritoneal exudate cells were stimulated by an intraperitoneal injection of 4% thioglycolate into male C57BL/6-Tg (CAG-EGFP)1Osb/J mice (the Jackson laboratory) aged 8 weeks. After 2 days, three million cells were injected intravenously into DKO-GFP or DKO-MYDGF mice that had been fed a WD for 12 weeks. After 2 days, aortic roots were embedded in OCT (optimal cutting temperature). Total fluorescent cells were counted in 10 × 8-mm sections over a 0.5-mm area, and the cell number was normalized to plaque area.
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Leukocyte Homing Assay in Mouse Atherosclerosis
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Aortic roots
Assay
Cells
Intraperitoneal injection
Leukocyte
Male
Mice
Peritoneal exudate
Plaque
Thioglycolate
Corresponding Organization : ICF International (United States)
Other organizations : Emory University
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Variable analysis
independent variables
- Feeding mice a Western diet (WD) for 12 weeks
- Total fluorescent cells counted in 10 × 8-mm sections over a 0.5-mm area
- Cell number normalized to plaque area
- Mouse strain (DKO-GFP or DKO-MYDGF)
- Age of mice (8 weeks)
- Gender of mice (male)
- Mouse strain (C57BL/6-Tg (CAG-EGFP)1Osb/J)
- Intraperitoneal injection of 4% thioglycolate to stimulate peritoneal exudate cells
- Injection of 3 million cells intravenously
- Positive control: Intraperitoneal injection of 4% thioglycolate to stimulate peritoneal exudate cells
- Negative control: Not explicitly mentioned
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