Protocol detail

Standardized Insect Photography Technique

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The photographs (Figs. 1, 2) were taken following the method described by Taszakowski and Kaszyca^{65 (link)}. To prepare high-quality photos that would enable advanced processing (e.g., obtaining a uniform background), the specimens were glued on transparent entomological glue boards and then cleaned with a thin brush. For better visibility of details, pictures of antennae were taken with the use of a dark background. The Focus-stacked, colour photographs were captured using the following equipment: Leica M205C (stereomicroscope), Leica LED5000 HDI (high diffuse dome illumination), Leica DFC495 (digital camera), Leica application suite 4.12.0 (software) (Leica Microsystems, Vienna, Austria), Image Composite Editor (panoramic image stitcher) and Adobe Photoshop CS6 graphic editor.Figure 2

Antennae of examined species in light microscopy and SEM: L. occidentalis (a, b) and L. zonatus (c, d).

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Taszakowski A., Masłowski A., Daane K.M, & Brożek J. (2023). Closer view of antennal sensory organs of two Leptoglossus species (Insecta, Hemiptera, Coreidae). Scientific Reports, 13, 617.

Publication 2023

LED5000 HDI Leica DFC495 Leica application suite 4.12.0 Image Composite Editor

Camera Figs Illumination Light microscopy

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Variable analysis

independent variables

The method described by Taszakowski and Kaszyca

dependent variables

High-quality photos that would enable advanced processing

control variables

Specimens were glued on transparent entomological glue boards
Pictures of antennae were taken with the use of a dark background

controls

No positive or negative controls were explicitly mentioned.

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