Multidrug Combination Therapy Evaluation

Doxorubicin (Dox, MB1087-1), Methotrexate (MTX, MB1156-1), Cisplatin (Cis, MB1055-1) and Paclitaxel (PTX, MB1178-1) were purchased from Meilunbio. Amiloride hydrochloride (PHR1839), NAC (A9165), DPI (43088), cyclosporin A (CsA, SML1018), EHT1864 (E1657), SKF-525A (567300), SB203580 (S8307), JSH-23 (J4455), PKH26-Red fluorescent cell linker kit, NH₄Cl (A9434), PKH67-Green fluorescent cell linker kit and anti-Lamp1 antibody (L1418) were purchased from Sigma-Aldrich. Ryanodine (ab120083), CGP37157 (ab120012) were purchased from Abcam. Bafilomycin A1 (HY-100558) were purchased from MCE. ER-, Mito-, Lyso-Tracker Green/Red, LysoSensor Yellow/Blue DND-160 (L7545), Early Endosomes-GFP (C10586), LysoSensor Green DND-189 (L7535), CellLight Golgi-RFP (C10593) and Late Endosomes-GFP (C10588) were purchased from Thermo Fisher Scientific. Clodronate liposomes were purchased from Liposomal.

Free full text: Click here

Wei K., Zhang H., Yang S., Cui Y., Zhang B., Liu J., Tang L., Tan Y., Liu S., Chen S., Yuan W., Luo X., Chen C., Li F., Liu J., Chen J., Xu P., Lv J., Tang K., Zhang Y., Ma J, & Huang B. (2023). Chemo-drugs in cell microparticles reset antitumor activity of macrophages by activating lysosomal P450 and nuclear hnRNPA2B1. Signal Transduction and Targeted Therapy, 8, 22.

Publication 2023

PKH67-Green fluorescent cell linker kit anti-Lamp1 antibody ( LysoSensor Yellow/Blue DND-160 LysoSensor Green DND-189 CellLight Golgi-RFP

Amiloride hydrochloride Anti antibody Bafilomycin a1 Cell Cgp37157 Cis 1 Cisplatin Clodronate Cyclosporin a Early endosomes Eht1864 Endosomes Golgi Jsh 23 Lamp1 Liposomal Lyso tracker Lysosensor green dnd 189 Lysosensor yellow blue Mito Paclitaxel Pkh26 Pkh67 Red cell Ryanodine Sb203580 Skf 525a

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, Union Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Wenzhou Medical University, First Affiliated Hospital of Wenzhou Medical University, First Affiliated Hospital of Zhengzhou University

Top 5 similar protocols

Variable analysis

independent variables

Doxorubicin (Dox, MB1087-1)
Methotrexate (MTX, MB1156-1)
Cisplatin (Cis, MB1055-1)
Paclitaxel (PTX, MB1178-1)
Amiloride hydrochloride (PHR1839)
NAC (A9165)
DPI (43088)
Cyclosporin A (CsA, SML1018)
EHT1864 (E1657)
SKF-525A (567300)
SB203580 (S8307)
JSH-23 (J4455)
Ryanodine (ab120083)
CGP37157 (ab120012)
Bafilomycin A1 (HY-100558)

dependent variables

Cell viability/cytotoxicity
Intracellular trafficking and localization of drugs/compounds

control variables

NH4Cl (A9434)
PKH26-Red fluorescent cell linker kit
PKH67-Green fluorescent cell linker kit
Anti-Lamp1 antibody (L1418)
ER-, Mito-, Lyso-Tracker Green/Red
LysoSensor Yellow/Blue DND-160 (L7545)
Early Endosomes-GFP (C10586)
LysoSensor Green DND-189 (L7535)
CellLight Golgi-RFP (C10593)
Late Endosomes-GFP (C10588)
Clodronate liposomes

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!