APOE Exon 4 Variant Analysis

The variants in exon four in the APOE gene (GenBank: NM_000041)—that is, c.388T > C and c.526C > T—were analyzed by polymerase chain reaction and Sanger sequencing in DNA extracted from peripheral leukocytes. Primers were designed using Primer3Plus (Bioin-formatics, Arlington, VA, USA) (34 (link)) and the polymerase chain reaction products were purified and Sanger sequenced using an ABI 3730xl DNA analyzer and an ABI BigDye terminator cycle-sequencing kit v3.1 (Thermo Fisher Scientific, Waltham, MA, USA). Sequences were analyzed with the DNA Sequencing Analysis software program (version 5.1; Applied Biosystems, Foster City, CA, USA) and the SeqScape software program (version 2.7; Thermo Fisher Scientific).

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Hellstrøm T., Andelic N., Holthe Ø.Ø., Helseth E., Server A., Eiklid K, & Sigurdardottir S. (2022). APOE-ε4 Is Associated With Reduced Verbal Memory Performance and Higher Emotional, Cognitive, and Everyday Executive Function Symptoms Two Months After Mild Traumatic Brain Injury. Frontiers in Neurology, 13, 735206.

Publication 2022

3730xl DNA analyzer ABI BigDye terminator cycle-sequencing kit v3.1 DNA Sequencing Analysis software program SeqScape software program

Abi 1 Apoe Dna sequencing analysis Exon Gene Leukocytes Polymerase chain reaction Primers

Corresponding Organization :

Other organizations : Oslo University Hospital, University of Oslo

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Variable analysis

independent variables

C.388T > C in exon four of the APOE gene
C.526C > T in exon four of the APOE gene

dependent variables

Genotypes of the variants c.388T > C and c.526C > T in exon four of the APOE gene

control variables

DNA extracted from peripheral leukocytes
Primers designed using Primer3Plus
Polymerase chain reaction
Sanger sequencing using an ABI 3730xl DNA analyzer and an ABI BigDye terminator cycle-sequencing kit v3.1
Sequence analysis using DNA Sequencing Analysis software (version 5.1) and SeqScape software (version 2.7)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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