Western Blot Protein Detection

According to a previous study [28 (link)], protein extracts were loaded onto 10% SDS gel and electrophoresed. The protein extracts were then transferred to the PVDF membrane (Millipore) and incubated with primary antibodies at 4°C overnight. The next day, the membrane was incubated with secondary antibodies for 2 h at room temperature. Finally, the protein bands were captured using ECL system (Thermo Fisher Scientific, Inc.).

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Liu H, & Wang X. (2022). Epidermal growth factor-like domain protein 6 recombinant protein facilitates osteogenic differentiation in adipose stem cells via bone morphogenetic protein 2/recombinant mothers against decapentaplegic homolog 4 signaling pathway. Bioengineered, 13(3), 6558-6566.

Publication 2022

PVDF membrane ECL system

Antibodies Membrane Protein Pvdf

Corresponding Organization : China Medical University

Other organizations : Jinzhou Central Hospital

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Variable analysis

independent variables

Protein extracts

dependent variables

Protein bands captured using ECL system

control variables

10% SDS gel
PVDF membrane (Millipore)
Primary antibodies
Secondary antibodies
Incubation at 4°C overnight
Incubation with secondary antibodies for 2 h at room temperature

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