MEFs and HEK293 and NMuMG cells were cultured in DMEM with 10% FBS. Primary wild-type or Mst1−/− MEFs were isolated, expanded, and cultured by conventional methods. Xtremegene HP (Roche) or Polythylenimine (PEI, Polyscience) transfection reagents was used for transfection. Infection of SeV, VSV, and HSV-1 was as described (Xu et al. 2014 (link)).
Protocol detail
Culturing and Transfecting Cell Lines
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Corresponding Organization :
Other organizations : Zhejiang University, Second Affiliated Hospital of Zhejiang University, University of California, San Diego, Baylor College of Medicine
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Variable analysis
independent variables
- Genotype (wild-type or Mst1^-/-)
- Unspecified
- Cell culture conditions (DMEM with 10% FBS)
- Transfection reagents (Xtremegene HP or Polythylenimine)
- Viral infections (SeV, VSV, and HSV-1)
- Positive control: Unspecified
- Negative control: Unspecified
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